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primepcr probe assay  (Bio-Rad)


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    Structured Review

    Bio-Rad primepcr probe assay
    Primepcr Probe Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primepcr+probe+assay/PrimePCR+Analysis+Software/pmc12814879-215-28-31
    Average 99 stars, based on 217 article reviews
    primepcr probe assay - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Labeling:

    Article Title: Versatile Genome Editing Using Type I-E CRISPR-Cas3 in Rice.
    Article Snippet: D ow nloaded from https://academ ic.oup.com /pcp/advance-article/doi/10.1093/pcp/pcaf138/8305110 by guest on 02 N ovem ber 2025 UN CO RR EC TE D MA NU SC RI PT ddPCR For ddPCR, 22 μl of reaction mixture containing 2.5 ng of genomic DNA digested with PacI and SacI, ddPCR Supermix for Probes (no dUTP) (Bio-Rad), and PrimePCR Probe Assay (Bio-Rad) were prepared. .. In this study, PrimePCR Probe Assay: ACT1, Rice labeled with HEX, PrimePCR Probe Assay: OS03G0183500 *, Rice labeled with FAM (–33.0 kb), PrimePCR Probe Assay: OS03G0183900 *, Rice labeled with FAM (–7.0 kb), PrimePCR Probe Assay: PDS, Rice labeled with FAM (–2.6 kb), and PrimePCR Probe Assay: OS03G0184100 *, Rice labeled with FAM (+6.0kb) were used (Bio-Rad). .. Droplet generation using an Automated Droplet Generator (Bio-Rad), PCR using droplets, droplet analysis using a QX200 Droplet Reader (Bio-Rad), and data analysis with QuantaSoft software (Bio-Rad) followed our previous report (Nishizawa-Yokoi et al. 2021).

    Article Title: Heritable Large Deletions Using Type I-E CRISPR-Cas3 in Rice
    Article Snippet: For ddPCR, 22 μl of reaction mixture containing 2.5 ng of genomic DNA digested with Pac I and Sac I, ddPCR Supermix for Probes (no dUTP) (Bio-Rad), and PrimePCR Probe Assay (Bio-Rad) were prepared. .. In this study, PrimePCR Probe Assay: ACT1, Rice labeled with HEX, PrimePCR Probe Assay: OS03G0183500 *, Rice labeled with FAM (–33.0 kb), PrimePCR Probe Assay: OS03G0183900 *, Rice labeled with FAM (–7.0 kb), PrimePCR Probe Assay: PDS, Rice labeled with FAM (–2.6 kb), and PrimePCR Probe Assay: OS03G0184100 *, Rice labeled with FAM (+6.0kb) were used (Bio-Rad). .. Droplet generation using an Automated Droplet Generator (Bio-Rad), PCR using droplets, droplet analysis using a QX200 Droplet Reader (Bio-Rad), and data analysis with QuantaSoft software (Bio-Rad) followed our previous report ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: A Cecal Slurry Mouse Model of Sepsis Leads to Acute Consumption of Vitamin C in the Brain
    Article Snippet: .. RNA was extracted using an RNeasy Mini Kit (Qiagen). qPCR was performed using a PrimePCR Probe Assay consisting of iScript cDNA synthesis, PrimePCR Probes (PrimePCRTM Probe Assay: Slc23a1, Mouse), and Sso Advanced Universal Supermix (Bio Rad). ..

    Article Title: Left-right side-specific endocrine signaling complements neural pathways to mediate acute asymmetric effects of brain injury
    Article Snippet: RNA (1 μg) was reverse-transcribed to cDNA with the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer's protocol. cDNA samples were aliquoted and stored at –20°C. .. TagMan assay in 384-well format was applied. cDNAs were mixed with PrimePCR Probe assay and iTaq Universal Probes supermix (Bio-Rad) for qPCR with a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, CA, USA) according to manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: A Cecal Slurry Mouse Model of Sepsis Leads to Acute Consumption of Vitamin C in the Brain
    Article Snippet: .. RNA was extracted using an RNeasy Mini Kit (Qiagen). qPCR was performed using a PrimePCR Probe Assay consisting of iScript cDNA synthesis, PrimePCR Probes (PrimePCRTM Probe Assay: Slc23a1, Mouse), and Sso Advanced Universal Supermix (Bio Rad). ..

    Quantitative RT-PCR:

    Article Title: Mesenchyme governs hair follicle induction
    Article Snippet: .. For embryonic skin samples treated with Rspo1, qRT-PCR was performed using gene-specific PrimePCR Probe Assay (Bio-Rad, Hercules, CA) for mouse Dkk4 , Fgf20 and Gapdh multiplexed in 20 μl reactions according to manufacturer’s instructions with 25 ng of template. .. Reactions were performed in triplicate wells with the CFX96 Real-Time System (Bio-Rad, Hercules, CA) with the protocol described earlier ( ).



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